Control:Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis , also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA ( 31 ). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 μm; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis, also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA (31). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 m; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
Infection:Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis , also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA ( 31 ). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 μm; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis, also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA (31). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 m; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
Enzyme-linked Immunosorbent Assay:Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis , also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA ( 31 ). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 μm; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
Article Title: Assessing the Inactivation of Mycobacterium avium subsp. paratuberculosis during Composting of Livestock Carcasses
Article Snippet: .. The four treatments examined included (i) a control using extra-lean ground beef, (ii) lab-cultured M. avium subsp. paratuberculosis (ATCC 19698), grown in Middlebrook 7H9 broth with albumin-dextrose-catalase (ADC) enrichment, inoculated onto ground beef, (iii) lab-cultured M. smegmatis (mc2 155; ATCC 700084), grown in the same type of culture medium as M. avium subsp. paratuberculosis, also inoculated onto ground beef, and (iv) lymph tissues (ileal, mesenteric, and ileocecal) collected from six cows infected with M. avium subsp. paratuberculosis as determined by serum ELISA (31). .. For each treatment, tissues (15 g) were mixed and placed into either a heat-sealed nylon bag (pore size, 50 m; Ankom rumen in situ bags; Ankom Technology, Macedon, NY), to assess the biochemical and microbial effects of composting on M. avium subsp. paratuberculosis viability, or into a sterile plastic bag to test the effects of temperature only.
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